FABI Articles : A Standardized Synthetic Eucalyptus Transcription Factor and Promoter Panel for Re-engineering Secondary Cell Wall Regulation in Biomass and Bioenergy Crops
previous pageA Standardized Synthetic Eucalyptus Transcription Factor and Promoter Panel for Re-engineering Secondary Cell Wall Regulation in Biomass and Bioenergy Crops
Steven
G. Hussey, Jacqueline Grima-Pettenati, Alexander A. Myburg, Eshchar
Mizrachi, Siobhan M. Brady, Yasuo Yoshikuni, and Samuel Deutsch
Abstract
Re-engineering
of transcriptional networks regulating secondary cell wall formation
may allow the improvement of plant biomass in widely grown plantation
crops such as Eucalyptus. However, there is currently a scarcity
of freely available standardized biological parts (e.g., Phytobricks)
compatible with Type IIS assembly approaches from forest trees, and
there is a need to accelerate transcriptional network inference in
nonmodel biomass crops. Here we describe the design and synthesis of a
versatile three-panel biological parts collection of 221 secondary cell
wall-related Eucalyptus grandis transcription factor coding
sequences and 65 promoters that are compatible with GATEWAY, Golden
Gate, MoClo, and GoldenBraid DNA assembly methods and generally conform
to accepted Phytobrick syntaxes. This freely available resource is
intended to accelerate synthetic biology applications in multiple plant
biomass crops and enable reconstruction of secondary cell wall
transcriptional networks using high-throughput assays such as DNA
affinity purification sequencing (DAP-seq) and enhanced yeast one-hybrid
(eY1H) screening.