Forestry in South Africa
Friday, August 12, 2022

click here to see
all logo's

FABI Articles : Botrytis eucalypti, a novel species isolated from diseased Eucalyptus seedlings in South China

previous page

Botrytis eucalypti, a novel species isolated from diseased Eucalyptus
seedlings in South China

Authors                            Authors and affiliations

QianLi Liu, GuoQing Li, JieQiong Li, ShuaiFei Chen

Original Article                                           Cite this article as:
First Online: 05 October 2016                      Liu, Q., Li, G., Li, J. et al. Mycol Progress 
DOI: 10.1007/s11557-016-1229-1              (2016). doi:10.1007/s11557-016-1229-1                             


Eucalyptus has become a preferred species for the production of industrial products and also for the protection of natural forests in South China. Many diseases affect these trees, both on plantations and in nurseries. One such disease in Eucalyptus nurseries is grey mould caused by a species of Botrytis. Symptoms of grey mould were recently observed on stems and leaves ofEucalyptus urophylla × Eucalyptus grandis seedlings in nurseries in ZhanJiang, GuangDong Province, South China. Diseased stems and leaves were covered with mycelium, conidiophores, and conidia of the causal pathogen. The fungus was identified on the basis of DNA sequence comparisons and morphological features, and its pathogenicity was tested on three Eucalyptusclones. Based on sequence comparisons of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA and partial DNA sequences of five nuclear gene regions, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), heat-shock protein 60 (HSP60), DNA-dependent RNA polymerase subunit II (RPB2), necrosis and ethylene-inducing proteins (NEP1and NEP2), combined with morphological characteristics and culture growth rate, the fungus represents a previously undescribed species of Botrytis. This undescribed species is phylogenetically and morphologically closely to Botrytis cinerea and B. pelargonii, but can be distinguished from them by DNA sequences of HSP60RPB2NEP1, and NEP2 gene regions, conidial characteristics, and culture growth rate. Here, a description of the fungus, designated as Botrytis eucalypti sp. nov., is provided. The results of in vitro leaf inoculation tests demonstrate that B. eucalypti is virulent on all three tested E. urophylla × E. grandis clones.

Read the paper HERE