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Post: LAMP assay to detect Elsinoë necatrix; an important Eucalyptus shoot and leaf pathogen

Eucalyptus

LAMP assay to detect Elsinoë necatrix; an important Eucalyptus shoot and leaf pathogen

Eucalyptus scab and shoot malformation caused by Elsinoë necatrix is an emerging disease and a serious threat to the global commercial forestry industry. The disease was first discovered in North Sumatra, Indonesia and now requires a simple and effective method for early pathogen detection. In this study, a rapid and sensitive Loop-mediated isothermal amplification (LAMP) assay was developed for E. necatrix. A unique region in a secondary metabolite gene cluster was used as target for the assay. To test robustness of the assay, LAMP amplification was verified in 15 strains of E. necatrix. A specificity test against 23 closely related Elsinoë species and three fungal species commonly isolated on Eucalyptus showed that the LAMP assay exclusively identified E. necatrix isolates. The assay had a high level of sensitivity, able to detect 0.01 ng (approximately 400 target copies) of pure E. necatrix DNA. Furthermore, using a simple DNA extraction method, it was possible to use this assay to detect E. necatrix in infected Eucalyptus leaves.

Read more: APS Journals

Source: APS Journals

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  • Husqvarna
  • John Deere
  • Khulani Timber Industries
  • Kwamahlati Training Services
  • LESH
  • Loadtech Load Cells
  • Logmech
  • Merensky
  • Mondi
  • Those who grow alone, die alone: why transformation is strategic for the MTO Group
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  • Ponsse
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  • Sabie Poles
  • SAFCOL
  • Sappi
  • Saw Specialists
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  • TWK
  • UCL Sawmill
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LAMP assay to detect Elsinoë necatrix; an important Eucalyptus shoot and leaf pathogen

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